Quidel MicroVue Serum PYD ELISA 提供了血清中吡啶啉交联 (Pyd) 排泄的定量测量。吡啶啉交联是 I 型胶原蛋白吸收的指标,尤其是骨胶原蛋白。
方法:竞争
分析物:吡啶啉
样品:血清
样品体积:25 µL 过滤后
LOD*:0.4 nmol/L
LLOQ*:0.75 nmol/L
HLOQ*:12 nmol/L
运行内精密 CV:6-15%
精密 CV运行间:9-12%
孵育时间:20-26 小时
交叉反应性:游离 PYD 99% 游离 DPD 0% PYD/DPD 肽 < 2.5% L-* 0.0004%(见包装插页)
*LOD = 检测限,LLOQ = 定量下限,HLOQ = 定量上限
96 孔微量滴定板,试剂足以重复检测 40 个样品
游离吡啶啉交联,与*和*有一些交叉反应(0.5 nmol/L 信号)
狒狒、猫、牛、狗、豚鼠、马、人、恒河猴、小鼠、大鼠
未检测到与兔反应
品牌 | 货号 | 品名 | 规格 |
---|---|---|---|
Quidel | 8019 | Quidel MicroVue Serum PYD ELISA | 96 Wells/Plate |
产品说明:
In humans, the total pool of urinary Pyd is approximately 45% free while the remaining fraction is bound to oligopeptides ranging from small linear peptides to very large cross-linked structures in excess of 10,000 Da. The proportion of free to total cross-links appears to be constant in healthy individuals and those with metabolic bone diseases or arthritis conditions, thus providing the rationale for measuring free Pyd.
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